Taxonomic assignment report

Facility	Hogwarts
Analyst	John Doe
Analysis started	2025-04-03 05:21:46
Analysis completed	2025-04-03 05:21:46
Wall time	0:0:0 hours

Sample details

locus	16S mitochondrial rRNA gene
preliminary_id	Trogoderma
taxa_of_interest	Trogoderma granarium
country	Pakistan
host	Rice
sample_id	VE25-1428_16S
Query DNA sequence

Result overview

Trogoderma granarium
Positive species identification.
Reasoning - Flag 1A: 1 candidate species matched with high stringency (identity ≥ 98.5%).

Preliminary morphology ID confirmed	True
Flag 7A: Candidate species is consistent with preliminary morphology ID

Taxa of interest ruled out	False
Flag 2B: Taxon of interest detected Flag 5.1C: The given locus for this taxon is not present in reference database (0 entries) Flag 5.2C: ≤10% of taxon have reference sequence(s) at the given locus

Analyst evaluation

Identification of candidate species

Flag 1A: Positive species identification - Trogoderma granarium
1 candidate species matched with high stringency (identity ≥ 98.5%)

Candidate hits must meet ONE of these criteria:

Minimum alignment length	`400bp`
Minimum query coverage	`85.0%`

Candidate hits are then classified as follows:

Classification	Alignment identity	Number of hits	Number of species
STRONG MATCH	≥ 98.5%	14	1
MODERATE MATCH	≥ 93.5%	NA	NA
NO MATCH	< 93.5%

Hits per candidate species (top 10 candidates only)

Species	Hits	Identity	E-value
Trogoderma granarium	14	100.0%	0.0

#	Accession	Hit subject	Align length	Query coverage	Score	E-value	Identity
1	MZ571637	Trogoderma granarium voucher DA336226 large subunit ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	427.0	0.00e+00	100.0%
2	KJ930431	Trogoderma granarium isolate der33 16S ribosomal RNA gene, partial sequence; mitochondrial	417	97.7%	417.0	0.00e+00	100.0%
3	MZ571636	Trogoderma granarium voucher DA335997 large subunit ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	424.0	0.00e+00	99.8%
4	KJ930432	Trogoderma granarium isolate der38 16S ribosomal RNA gene, partial sequence; mitochondrial	417	97.7%	414.0	0.00e+00	99.8%
5	OM388538	Trogoderma granarium isolate NNM37 large subunit ribosomal RNA gene, partial sequence; mitochondrial	393	92.0%	390.0	0.00e+00	99.7%
6	KJ930487	Trogoderma granarium isolate der226 16S ribosomal RNA gene, partial sequence; mitochondrial	352	82.4%	349.0	1.50e-180	99.7%
7	MN535884	Trogoderma granarium isolate TR1 large subunit ribosomal RNA gene, partial sequence; mitochondrial	248	58.1%	245.0	9.76e-123	99.6%
8	LC386208	Trogoderma granarium mitochondrial DNA, contig: 140401 T. granarium Contig1	427	100.0%	421.0	0.00e+00	99.5%
9	ON725093	Trogoderma granarium isolate 1RC large subunit ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	421.0	0.00e+00	99.5%
10	NC_053875	Trogoderma granarium mitochondrion, complete genome	427	100.0%	421.0	0.00e+00	99.5%
11	KJ930452	Trogoderma granarium isolate der88 16S ribosomal RNA gene, partial sequence; mitochondrial	417	97.7%	411.0	0.00e+00	99.5%
12	KJ930433	Trogoderma granarium isolate der39 16S ribosomal RNA gene, partial sequence; mitochondrial	417	97.7%	411.0	0.00e+00	99.5%
13	OM389182	Trogoderma granarium isolate NNM57 large subunit ribosomal RNA gene, partial sequence; mitochondrial	392	91.8%	386.0	0.00e+00	99.5%
14	OM388512	Trogoderma granarium isolate nnm6 large subunit ribosomal RNA gene, partial sequence; mitochondrial	383	89.7%	377.0	0.00e+00	99.5%
15	MW911680	Trogoderma granarium isolate 1594 large subunit ribosomal RNA gene, partial sequence; mitochondrial	208	48.7%	205.0	1.68e-100	99.5%
16	OM388567	Trogoderma granarium isolate partial 46 large subunit ribosomal RNA gene, partial sequence; mitochondrial	393	92.0%	384.0	0.00e+00	99.2%
17	OM389131	Trogoderma granarium isolate partial 14 large subunit ribosomal RNA gene, partial sequence; mitochondrial	392	91.8%	383.0	0.00e+00	99.2%
18	MW911673	Trogoderma granarium isolate 1610 large subunit ribosomal RNA gene, partial sequence; mitochondrial	208	48.7%	202.0	7.82e-99	99.0%
19	MW911674	Trogoderma granarium isolate 1609 large subunit ribosomal RNA gene, partial sequence; mitochondrial	205	48.0%	199.0	3.64e-97	99.0%
20	MW911689	Trogoderma granarium isolate 1631 large subunit ribosomal RNA gene, partial sequence; mitochondrial	182	42.6%	176.0	2.22e-84	98.9%
21	MW911686	Trogoderma granarium isolate 1583 large subunit ribosomal RNA gene, partial sequence; mitochondrial	208	48.7%	199.0	3.64e-97	98.6%
22	MW911679	Trogoderma granarium isolate 1598 large subunit ribosomal RNA gene, partial sequence; mitochondrial	208	48.7%	196.0	1.69e-95	98.1%
23	MW049034	Trogoderma granarium strain TR4 large subunit ribosomal RNA gene, partial sequence; mitochondrial	214	50.1%	201.0	2.81e-98	97.7%
24	MW049033	Trogoderma granarium strain TR5 large subunit ribosomal RNA gene, partial sequence; mitochondrial	214	50.1%	197.0	4.71e-96	97.2%
25	MZ571638	Trogoderma glabrum voucher VAITC10096 large subunit ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	348.0	5.40e-180	93.9%
26	KJ930458	Trogoderma glabrum isolate der105 16S ribosomal RNA gene, partial sequence; mitochondrial	417	97.7%	338.0	1.96e-174	93.8%
27	KJ930508	Trogoderma glabrum isolate der259 16S ribosomal RNA gene, partial sequence; mitochondrial	401	93.9%	325.0	3.29e-167	93.8%
28	KJ930455	Trogoderma inclusum isolate der94 16S ribosomal RNA gene, partial sequence; mitochondrial	417	97.7%	257.0	2.08e-129	87.4%
29	MZ571643	Trogoderma sp. VAITC9052 large subunit ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	258.0	5.79e-130	87.0%
30	PP239566	Trogoderma versicolor breed T4 large subunit ribosomal RNA gene, partial sequence; mitochondrial	424	99.3%	255.0	2.70e-128	86.9%
31	PP239565	Trogoderma versicolor breed T3 large subunit ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	255.0	2.70e-128	86.7%
32	KJ930507	Trogoderma simplex isolate der254 16S ribosomal RNA gene, partial sequence; mitochondrial	417	97.7%	240.0	5.88e-120	86.0%
33	KJ930462	Trogoderma grassmani isolate der131 16S ribosomal RNA gene, partial sequence; mitochondrial	408	95.6%	225.0	1.28e-111	85.3%
34	KJ930515	Trogoderma sternale plagifer isolate der280 16S ribosomal RNA gene, partial sequence; mitochondrial	407	95.3%	224.0	4.61e-111	85.3%
35	KJ930460	Trogoderma sternale sternale isolate der116 16S ribosomal RNA gene, partial sequence; mitochondrial	408	95.6%	224.0	4.61e-111	85.2%
36	KJ930444	Trogoderma variabile isolate der68 16S ribosomal RNA gene, partial sequence; mitochondrial	407	95.3%	223.0	1.66e-110	85.2%
37	KJ930504	Trogoderma sternale isolate der251 16S ribosomal RNA gene, partial sequence; mitochondrial	408	95.6%	222.0	5.96e-110	85.1%
38	KJ930467	Trogoderma teukton isolate der177 16S ribosomal RNA gene, partial sequence; mitochondrial	417	97.7%	224.0	4.61e-111	84.8%
39	KJ930469	Trogoderma grassmani isolate der184 16S ribosomal RNA gene, partial sequence; mitochondrial	408	95.6%	219.0	2.77e-108	84.8%
40	KJ930472	Trogoderma ornatum isolate der196 16S ribosomal RNA gene, partial sequence; mitochondrial	405	94.8%	215.0	4.64e-106	84.7%
41	KJ930475	Trogoderma sternale sternale isolate der201 16S ribosomal RNA gene, partial sequence; mitochondrial	387	90.6%	208.0	3.61e-102	84.7%
42	KJ930501	Trogoderma anthrenoides isolate der248 16S ribosomal RNA gene, partial sequence; mitochondrial	358	83.8%	188.0	4.74e-91	84.6%
43	KJ930509	Trogoderma sternale plagifer isolate der262 16S ribosomal RNA gene, partial sequence; mitochondrial	407	95.3%	215.0	4.64e-106	84.5%
44	KJ930476	Trogoderma ornatum isolate der202 16S ribosomal RNA gene, partial sequence; mitochondrial	405	94.8%	212.0	2.16e-104	84.5%
45	KJ930499	Trogoderma ornatum isolate der244 16S ribosomal RNA gene, partial sequence; mitochondrial	405	94.8%	212.0	2.16e-104	84.5%
46	GQ981024	Atheta laticollis voucher ZMUN:10002606 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	193.0	7.87e-94	84.5%
47	MZ571639	Trogoderma variabile voucher VAITC9461 large subunit ribosomal RNA gene, partial sequence; mitochondrial	380	89.0%	196.0	1.69e-95	84.2%
48	MT571479	Troglocharinus sendrai voucher IBE_AN491 large subunit ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	195.0	6.09e-95	84.1%
49	MT571478	Troglocharinus sendrai voucher IBE_AN351 large subunit ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	195.0	6.09e-95	84.1%
50	MT571476	Troglocharinus mercedesi voucher IBE_RA462 large subunit ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	195.0	6.09e-95	84.1%
51	MT571475	Troglocharinus mercedesi voucher IBE_PB33 large subunit ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	194.0	2.19e-94	84.1%
52	MK234421	Psammostiba hilleri isolate CNUIC0227 16S ribosomal RNA gene, partial sequence; mitochondrial	365	85.5%	187.0	1.70e-90	84.0%
53	KJ930429	Megatoma sp. der31 16S ribosomal RNA gene, partial sequence; mitochondrial	409	95.8%	207.0	1.30e-101	83.9%
54	MT571468	Stygiophyes puncticollis voucher IBE_RA932 large subunit ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	192.0	2.83e-93	83.9%
55	LN849364	Stygiophyes zariquieyi genomic DNA containing rrnL-trnL-nad1 region, specimen voucher IBE-AF187	377	88.3%	192.0	2.83e-93	83.9%
56	JN814894	Enochrus bicolor isolate IBE-AB287 16S ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	363	85.0%	184.0	7.93e-89	83.8%
57	JN814905	Enochrus bicolor isolate IBE-AB59 16S ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	363	85.0%	184.0	7.93e-89	83.8%
58	JN814898	Enochrus bicolor isolate IBE-AB328 16S ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	363	85.0%	184.0	7.93e-89	83.8%
59	JN814899	Enochrus bicolor isolate IBE-AB329 16S ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	363	85.0%	184.0	7.93e-89	83.8%
60	JN814907	Enochrus bicolor isolate IBE-AB7 16S ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	363	85.0%	184.0	7.93e-89	83.8%
61	JN814901	Enochrus bicolor isolate IBE-AB39 16S ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	363	85.0%	184.0	7.93e-89	83.8%
62	JN814896	Enochrus bicolor isolate IBE-AB29 16S ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	363	85.0%	184.0	7.93e-89	83.8%
63	JN814880	Enochrus bicolor isolate IBE-AB228 16S ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	363	85.0%	184.0	7.93e-89	83.8%
64	JN814884	Enochrus bicolor isolate IBE-AB232 16S ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	363	85.0%	184.0	7.93e-89	83.8%
65	KJ930428	Trogoderma sternale isolate der30 16S ribosomal RNA gene, partial sequence; mitochondrial	387	90.6%	194.0	2.19e-94	83.7%
66	LN849373	Stygiophyes latebricola elongatus genomic DNA containing rrnL-trnL-nad1 region, specimen voucher MNCN-AI596	377	88.3%	190.0	3.66e-92	83.7%
67	GU356783	Salgadoia brieti 16S ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	189.0	1.32e-91	83.7%
68	AM229397	Stygiophyes ribagorzanus mitochondrial 16S rRNA gene	374	87.6%	188.0	4.74e-91	83.7%
69	MK234430	Saphocallus parviceps isolate CNUIC0550 16S ribosomal RNA gene, partial sequence; mitochondrial	358	83.8%	181.0	3.69e-87	83.7%
70	KJ930473	Trogoderma fasciferum isolate der199 16S ribosomal RNA gene, partial sequence; mitochondrial	379	88.8%	190.0	3.66e-92	83.6%
71	MT571477	Troglocharinus mercedesi voucher IBE_AN1248 large subunit ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	189.0	1.32e-91	83.6%
72	HG915672	Leonesiella bergidi mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher MNCN:AC59	377	88.3%	189.0	1.32e-91	83.6%
73	HG915708	Stygiophyes aldomai allomorphus mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher MNCN:AI1077	377	88.3%	188.0	4.74e-91	83.6%
74	LN849368	Stygiophyes ribagorzanus genomic DNA containing rrnL region, specimen voucher MNCN-AI575	377	88.3%	188.0	4.74e-91	83.6%
75	GU356765	Lagariella colominasi 16S ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	188.0	4.74e-91	83.6%
76	GQ981016	Atheta dadopora voucher ZMUN:10002554 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	184.0	7.93e-89	83.6%
77	GQ981023	Atheta subtilis voucher ZMUN:10002566 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	183.0	2.85e-88	83.6%
78	JN814893	Enochrus bicolor isolate IBE-AB286 16S ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	363	85.0%	181.0	3.69e-87	83.6%
79	MK234423	Psammostiba kenaii isolate CNUIC0274 16S ribosomal RNA gene, partial sequence; mitochondrial	365	85.5%	181.0	3.69e-87	83.6%
80	JN814903	Enochrus bicolor isolate IBE-AB43 16S ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	363	85.0%	181.0	3.69e-87	83.6%
81	JN814904	Enochrus bicolor isolate IBE-AB58 16S ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	363	85.0%	181.0	3.69e-87	83.6%
82	KJ930470	Trogoderma fasciferum isolate der193 16S ribosomal RNA gene, partial sequence; mitochondrial	400	93.7%	199.0	3.64e-97	83.5%
83	LT797425	Troglocharinus kiesenwetteri mitochondrial genomic DNA containing rrnL-trnL-nad1 region, specimen voucher IBE-RA899	377	88.3%	186.0	6.13e-90	83.5%
84	HF912610	Troglocharinus ferreri ferreri mitochondrial partial 16S rRNA gene, specimen voucher IBE	377	88.3%	186.0	6.13e-90	83.5%
85	HG915701	Speonomus sp. IR-2014 mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher IBE	377	88.3%	186.0	6.13e-90	83.5%
86	HF912621	Troglocharinus kiesenwetteri sanllorensi mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher IBE	377	88.3%	186.0	6.13e-90	83.5%
87	HF912624	Troglocharinus kiesenwetteri sanllorensi mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher IBE	377	88.3%	186.0	6.13e-90	83.5%
88	HF912636	Troglocharinus elongatus pinyareti mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher IBE	377	88.3%	186.0	6.13e-90	83.5%
89	HF912622	Troglocharinus kiesenwetteri sanllorensi mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher IBE	377	88.3%	186.0	6.13e-90	83.5%
90	HF912629	Troglocharinus jacasi mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher IBE	377	88.3%	186.0	6.13e-90	83.5%
91	HF912612	Troglocharinus ferreri ferreri mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher MNCN:AI1065	377	88.3%	185.0	2.20e-89	83.5%
92	HF912623	Troglocharinus kiesenwetteri andresi mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher IBE	377	88.3%	185.0	2.20e-89	83.5%
93	HF912633	Troglocharinus ferreri mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher IBE	377	88.3%	185.0	2.20e-89	83.5%
94	KP682745	Bruchidius mulunguensis voucher BRU.GK21 16S ribosomal RNA gene, partial sequence; mitochondrial	366	85.7%	182.0	1.03e-87	83.5%
95	MK234405	Adota maritima isolate CNUIC0488 16S ribosomal RNA gene, partial sequence; mitochondrial	365	85.5%	182.0	1.03e-87	83.5%
96	KP682744	Bruchidius mulunguensis voucher BRU.GK167 16S ribosomal RNA gene, partial sequence; mitochondrial	366	85.7%	181.0	3.69e-87	83.5%
97	JX536452	Neothetalia nimia voucher ZMUN:10029172 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	181.0	3.69e-87	83.5%
98	HF912616	Speonomus zophosinus mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher MNCN:AI667	377	88.3%	186.0	6.13e-90	83.4%
99	AM229404	Speonomus hydrophilus mitochondrial 16S rRNA gene	377	88.3%	186.0	6.13e-90	83.4%
100	HG915704	Speonomus normandi normandi mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher MNCN:AI532	377	88.3%	186.0	6.13e-90	83.4%
101	HG915703	Speonomus normandi hydrophilus mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher MNCN:AI528	377	88.3%	186.0	6.13e-90	83.4%
102	KJ930505	Trogoderma sternale aspericolle isolate der252 16S ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	201.0	2.81e-98	83.3%
103	MT571481	Stygiophyes latebricola latebricola voucher IBE_AN339 large subunit ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	186.0	6.13e-90	83.3%
104	MT571467	Stygiophyes saforensis voucher IBE_AC105 large subunit ribosomal RNA (rrnL) and tRNA-Leu (trnL) genes, partial sequence; mitochondrial	377	88.3%	185.0	2.20e-89	83.3%
105	KJ930474	Trogoderma fasciferum isolate der200 16S ribosomal RNA gene, partial sequence; mitochondrial	400	93.7%	196.0	1.69e-95	83.2%
106	HG915706	Speonomus pyreneus major mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher MNCN:AI535	377	88.3%	183.0	2.85e-88	83.2%
107	MT571485	Speonomus pyreneus pyreneus voucher MNCN_AC55 large subunit ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	183.0	2.85e-88	83.2%
108	LT797426	Troglocharinus pallisei mitochondrial genomic DNA containing rrnL-trnL-nad1 region, specimen voucher IBE-RA1190	377	88.3%	183.0	2.85e-88	83.2%
109	LT797428	Troglocharinus orcinus mitochondrial genomic DNA containing rrnL-trnL-nad1 region, specimen voucher IBE-VR20	377	88.3%	183.0	2.85e-88	83.2%
110	HF912606	Troglocharinus elongatus mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher IBE	377	88.3%	183.0	2.85e-88	83.2%
111	HG915705	Speonomus pyreneus mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher MNCN:AI668	377	88.3%	183.0	2.85e-88	83.2%
112	GU356794	Speonomus diecki 16S ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	183.0	2.85e-88	83.2%
113	GU356796	Speonomus orgibetensis 16S ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	183.0	2.85e-88	83.2%
114	HG915698	Speonomus bastideus mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher IBE	377	88.3%	183.0	2.85e-88	83.2%
115	LT797427	Troglocharinus pallisei mitochondrial genomic DNA containing rrnL-trnL-nad1 region, specimen voucher IBE-RA1191	377	88.3%	183.0	2.85e-88	83.2%
116	HF912620	Troglocharinus orcinus mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher IBE	377	88.3%	183.0	2.85e-88	83.2%
117	HF912642	Troglocharinus orcinus orcinus mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher IBE	377	88.3%	183.0	2.85e-88	83.2%
118	HF912626	Troglocharinus patracoi mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher IBE	377	88.3%	182.0	1.03e-87	83.2%
119	GQ981032	Atheta graminicola voucher ZMUN:10002562 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	178.0	1.72e-85	83.2%
120	JX536453	Ocalea badia voucher ZMUN:10002740 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	178.0	1.72e-85	83.2%
121	KF755232	Enochrus coarctatus voucher IBE	362	84.8%	177.0	6.17e-85	83.2%
122	GQ981017	Atheta aeneipennis voucher ZMUN:10002583 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	402	94.1%	194.0	2.19e-94	83.1%
123	MH317867	Oocyclus sp. SLE0933 16S ribosomal RNA gene, partial sequence; mitochondrial	376	88.1%	182.0	1.03e-87	83.1%
124	MH317857	Oocyclus meridensis isolate SLE0038 16S ribosomal RNA gene, partial sequence; mitochondrial	371	86.9%	180.0	1.33e-86	83.1%
125	JN581714	Drusilla sp. 1 HE-2012 voucher ZMUN:10051251 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	398	93.2%	192.0	2.83e-93	83.0%
126	GQ981037	Boreophilia hyperborea voucher ZMUN:10002634 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	395	92.5%	191.0	1.02e-92	83.0%
127	KF625966	Lampyridae sp. UPOL RK0390 16S ribosomal RNA gene, partial sequence; mitochondrial	385	90.2%	186.0	6.13e-90	83.0%
128	HF912614	Troglocharinus senenti mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher MNCN:AI585	377	88.3%	180.0	1.33e-86	83.0%
129	MT571480	Troglocharinus vinyasi voucher IBE_AN790 large subunit ribosomal RNA (rrnL) and tRNA-Leu (trnL) genes, partial sequence; mitochondrial	377	88.3%	180.0	1.33e-86	83.0%
130	HF912625	Troglocharinus elongatus ollai mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher IBE	377	88.3%	180.0	1.33e-86	83.0%
131	JX412837	Attagenus hottentotus mitochondrion, partial genome	373	87.4%	179.0	4.77e-86	83.0%
132	MK234413	Atheta tokiokai isolate CNUIC0492 16S ribosomal RNA gene, partial sequence; mitochondrial	365	85.5%	175.0	7.99e-84	83.0%
133	NC_028598	Callicerus obscurus mitochondrion, complete genome	409	95.8%	195.0	6.09e-95	82.9%
134	KJ930456	Trogoderma simplex isolate der98 16S ribosomal RNA gene, partial sequence; mitochondrial	407	95.3%	194.0	2.19e-94	82.9%
135	GQ980987	Pella caliginosa voucher ZMUN:10002616 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	402	94.1%	194.0	2.19e-94	82.9%
136	JN581713	Drusilla sp. 1 HE-2012 voucher ZMUN:10051252 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	406	95.1%	194.0	2.19e-94	82.9%
137	GQ980986	Pella caliginosa voucher ZMUN:10002617 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	398	93.2%	193.0	7.87e-94	82.9%
138	HE663530	Cantabrogeus luquei mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and nad1 gene (partial), specimen voucher IBE-AF3	377	88.3%	180.0	1.33e-86	82.9%
139	GU356797	Speonomus stygius stygius 16S ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	180.0	1.33e-86	82.9%
140	HG915710	Troglodromus bucheti mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher IBE	377	88.3%	180.0	1.33e-86	82.9%
141	KC992697	Oocyclus sapphirus 16S ribosomal RNA gene, partial sequence	376	88.1%	179.0	4.77e-86	82.9%
142	PP474516	Attagenus smirnovi voucher AS16 large subunit ribosomal RNA gene, partial sequence; mitochondrial	405	94.8%	192.0	2.83e-93	82.8%
143	PP474517	Attagenus smirnovi voucher A17 large subunit ribosomal RNA gene, partial sequence; mitochondrial	405	94.8%	192.0	2.83e-93	82.8%
144	LT797424	Troglocharinus subilsi mitochondrial genomic DNA containing rrnL-trnL-nad1 region, specimen voucher IBE-RA1189	377	88.3%	177.0	6.17e-85	82.8%
145	NC_032062	Asymmetricata circumdata mitochondrion, complete genome	374	87.6%	176.0	2.22e-84	82.8%
146	MT187623	Iotarphia rufobrunnea isolate Cloudy Bay, Bruny Is, Tasmania, Australia large subunit ribosomal RNA gene, partial sequence; mitochondrial	365	85.5%	175.0	7.99e-84	82.8%
147	KC964533	Pleophylla sp. BNHM835042 16S ribosomal RNA gene, partial sequence; mitochondrial	367	85.9%	175.0	7.99e-84	82.8%
148	KT303874	Pleophylla pilosa voucher ZFMK:COL:DA1671 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	367	85.9%	175.0	7.99e-84	82.8%
149	KC964472	Pleophylla sp. BNHM834734 16S ribosomal RNA gene, partial sequence; mitochondrial	367	85.9%	174.0	2.87e-83	82.8%
150	GQ980998	Mocyta scopula voucher ZMUN:10002559 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	173.0	1.03e-82	82.8%
151	GQ980997	Mocyta scopula voucher ZMUN:10002540 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	173.0	1.03e-82	82.8%
152	GQ981013	Atheta modesta voucher ZMUN:10002620 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	173.0	1.03e-82	82.8%
153	JN581757	Pedinopleurus sp. HE-2012 voucher ZMUN:10051235 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	413	96.7%	196.0	1.69e-95	82.7%
154	JN581709	Callicerus obscurus voucher ZMUN:10030905 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	412	96.5%	195.0	6.09e-95	82.7%
155	PP474515	Attagenus smirnovi voucher AS7 large subunit ribosomal RNA gene, partial sequence; mitochondrial	403	94.4%	190.0	3.66e-92	82.7%
156	KT303914	Trochalus sp. DA1518 voucher ZFMK:COL:DA1518 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	377	88.3%	178.0	1.72e-85	82.7%
157	GQ980994	Lypoglossa lateralis voucher ZMUN:10002632 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	372	87.1%	177.0	6.17e-85	82.7%
158	HG915707	Speonomus speluncarum mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher IBE	377	88.3%	177.0	6.17e-85	82.7%
159	KC964439	Pleophylla sp. BNHM834701 16S ribosomal RNA gene, partial sequence; mitochondrial	367	85.9%	175.0	7.99e-84	82.7%
160	GQ981031	Atheta graminicola voucher ZMUN:10002561 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	174.0	2.87e-83	82.7%
161	GQ981005	Atheta gagatina voucher ZMUN:10002578 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	172.0	3.72e-82	82.7%
162	GQ981006	Atheta gagatina voucher ZMUN:10002580 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	172.0	3.72e-82	82.7%
163	KF755280	Enochrus (Methydrus) sp. IBE-AB55 voucher IBE	362	84.8%	172.0	3.72e-82	82.7%
164	MK234412	Atheta tokiokai isolate CNUIC0233 16S ribosomal RNA gene, partial sequence; mitochondrial	365	85.5%	172.0	3.72e-82	82.7%
165	GU356798	Stygiophyes akarsticus 16S ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	358	83.8%	170.0	4.81e-81	82.7%
166	NC_063826	Anthrenus museorum mitochondrion, complete genome	422	98.8%	196.0	1.69e-95	82.6%
167	MZ571644	Anthrenus verbasci voucher VAITC9639 large subunit ribosomal RNA gene, partial sequence; mitochondrial	422	98.8%	196.0	1.69e-95	82.6%
168	KX087239	Anthrenus verbasci mitochondrion, partial genome	422	98.8%	196.0	1.69e-95	82.6%
169	KJ930510	Trogoderma sternale aspericolle isolate der270 16S ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	192.0	2.83e-93	82.6%
170	JN581691	Alevonota egregia voucher ZMUN:10030891 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	404	94.6%	190.0	3.66e-92	82.6%
171	KX092728	Sostea sp. UPOL RK0688 16S ribosomal RNA gene, partial sequence; mitochondrial	402	94.1%	189.0	1.32e-91	82.6%
172	DQ202600	Frickius variolosus voucher BMNH:679317 16S ribosomal RNA gene, partial sequence; mitochondrial	401	93.9%	188.0	4.74e-91	82.6%
173	JN097742	Anthrenus sp. KY KLB-2011 16S ribosomal RNA gene, partial sequence; mitochondrial	394	92.3%	183.0	2.85e-88	82.6%
174	GU356770	Paranillochlamys velox voucher NHM-IRC37 16S ribosomal RNA gene, partial sequence; mitochondrial	386	90.4%	182.0	1.03e-87	82.6%
175	MT571470	Speonomus leleupi voucher IBE_AN414 large subunit ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	177.0	6.17e-85	82.6%
176	HE663533	Quaestus pasensis mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and nad1 gene (partial), specimen voucher IBE-AC97	377	88.3%	177.0	6.17e-85	82.6%
177	DQ680896	Onthophagus sp. BM670919 16S ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	367	85.9%	173.0	1.03e-82	82.6%
178	JX536454	Ocalea corsicana voucher ZMUN:10008453 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	172.0	3.72e-82	82.6%
179	KC992701	Paracymus limbatus 16S ribosomal RNA gene, partial sequence	374	87.6%	175.0	7.99e-84	82.5%
180	AM229673	Speonomus longicornis partial mitochondrial 16S rRNA gene	374	87.6%	174.0	2.87e-83	82.5%
181	KC992699	Oocyclus trujillo 16S ribosomal RNA gene, partial sequence	371	86.9%	171.0	1.34e-81	82.5%
182	EU477776	Adinopsis sp. 1 JCT-2008 16S ribosomal RNA gene, partial sequence; mitochondrial	365	85.5%	170.0	4.81e-81	82.5%
183	KY495955	Dorylophila sp. JP-2017 16S ribosomal RNA gene, partial sequence; mitochondrial	415	97.2%	192.0	2.83e-93	82.4%
184	JN581690	Alevonota egregia voucher ZMUN:10030889 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	414	97.0%	192.0	2.83e-93	82.4%
185	MT012204	Cafius mutatus isolate Monterey, California, USA large subunit ribosomal RNA gene, partial sequence; mitochondrial	414	97.0%	191.0	1.02e-92	82.4%
186	DQ198671	Curtos sp. UPOL 000M16 16S ribosomal RNA gene, partial sequence; mitochondrial	385	90.2%	178.0	1.72e-85	82.4%
187	MZ571657	Dermestidae sp. VAITC9100 large subunit ribosomal RNA gene, partial sequence; mitochondrial	377	88.3%	175.0	7.99e-84	82.4%
188	HG915702	Speonomus longicornis longicornis mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher MNCN:AI531	377	88.3%	174.0	2.87e-83	82.4%
189	KT303909	Pleophylla sp. DA3642 voucher ZFMK:COL:DA3642 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	367	85.9%	171.0	1.34e-81	82.4%
190	MK234415	Iotarphia australis isolate CNUIC0329 16S ribosomal RNA gene, partial sequence; mitochondrial	365	85.5%	170.0	4.81e-81	82.4%
191	JX536443	Ilyobates nigricollis voucher ZMUN:10030926 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	170.0	4.81e-81	82.4%
192	JX536484	Stenectinobregma sp. ZMUN 10008435 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	169.0	1.73e-80	82.4%
193	MK234428	Tarphiota geniculata isolate CNUIC0493 16S ribosomal RNA gene, partial sequence; mitochondrial	365	85.5%	169.0	1.73e-80	82.4%
194	JX536455	Ocalea vancouveri voucher ZMUN:10029146 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	169.0	1.73e-80	82.4%
195	JX536462	Oxypoda opaca voucher ZMUN:10002708 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	365	85.5%	169.0	1.73e-80	82.4%
196	KF755283	Enochrus testaceus voucher IBE	363	85.0%	169.0	1.73e-80	82.4%
197	KF755282	Enochrus testaceus voucher IBE	363	85.0%	169.0	1.73e-80	82.4%
198	MK234427	Tarphiota geniculata isolate CNUIC0069 16S ribosomal RNA gene, partial sequence; mitochondrial	365	85.5%	169.0	1.73e-80	82.4%
199	JN814900	Enochrus testaceus isolate IBE-AB36 16S ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	363	85.0%	169.0	1.73e-80	82.4%
200	OM502078	Pseudodisersus goudotii voucher IBSAS-FZ0622 16S ribosomal RNA gene, partial sequence; mitochondrial	363	85.0%	169.0	1.73e-80	82.4%
201	KF755284	Enochrus testaceus voucher IBE	363	85.0%	169.0	1.73e-80	82.4%
202	KF755281	Enochrus testaceus voucher IBE	363	85.0%	169.0	1.73e-80	82.4%
203	GQ981018	Atheta cinnamoptera voucher ZMUN:10002582 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	402	94.1%	185.0	2.20e-89	82.3%
204	GQ981034	Atheta vaga voucher ZMUN:10002655 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	395	92.5%	181.0	3.69e-87	82.3%
205	JN581722	Ecitomorpha sp. HE-2012 voucher ZMUN:10002689 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	380	89.0%	175.0	7.99e-84	82.3%
206	LN849369	Trapezodirus arcticollis genomic DNA containing rrnL-trnL-nad1 region, specimen voucher MNCN-AI576	377	88.3%	170.0	4.81e-81	82.3%
207	HF912615	Trapezodirus cerberus mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher MNCN:AI600	377	88.3%	170.0	4.81e-81	82.3%
208	AY131467	Monoplistes curvipes 16S ribosomal RNA gene, partial sequence; mitochondrial	417	97.7%	191.0	1.02e-92	82.2%
209	HF912617	Troglocharinus impellitierii mitochondrial partial 16S rRNA gene, specimen voucher NHMUK:IRC22	411	96.3%	186.0	6.13e-90	82.2%
210	MH317859	Oocyclus nr. petra SLE0467 16S ribosomal RNA gene, partial sequence; mitochondrial	407	95.3%	185.0	2.20e-89	82.2%
211	AB178317	Phelotrupes laevistriatus mitochondrial gene for 16S rRNA, partial sequence	386	90.4%	178.0	1.72e-85	82.2%
212	GU356784	Spelaeochlamys ehlersi voucher NHM-IRC31 16S ribosomal RNA gene, partial sequence; mitochondrial	386	90.4%	178.0	1.72e-85	82.2%
213	KC992709	Spercheus emarginatus 16S ribosomal RNA gene, partial sequence	373	87.4%	172.0	3.72e-82	82.2%
214	AM287063	Spercheus emarginatus mitochondrial partial 16S rRNA gene	373	87.4%	172.0	3.72e-82	82.2%
215	JN581728	Geostiba nubigena voucher ZMUN:10030736 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	406	95.1%	184.0	7.93e-89	82.1%
216	JN581727	Geostiba nubigena voucher ZMUN:10030888 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	406	95.1%	184.0	7.93e-89	82.1%
217	JN581737	Lomechusa pubicollis voucher ZMUN:10030917 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	405	94.8%	184.0	7.93e-89	82.1%
218	MN053053	Epilachna admirabilis mitochondrion, partial genome	402	94.1%	184.0	7.93e-89	82.1%
219	HQ178221	Bruchidius cinerascens voucher BRU-Cd11 16S ribosomal RNA gene, partial sequence; mitochondrial	400	93.7%	181.0	3.69e-87	82.1%
220	OR076755	Hydora musci voucher H3.1 large subunit ribosomal RNA gene, partial sequence; mitochondrial	377	88.3%	172.0	3.72e-82	82.1%
221	AY131466	Monoplistes sp. dgi1 16S ribosomal RNA gene, partial sequence; mitochondrial	417	97.7%	187.0	1.70e-90	82.0%
222	AM287070	Hydrobius fuscipes mitochondrial partial 16S rRNA gene	413	96.7%	183.0	2.85e-88	82.0%
223	FJ903703	Formicomus pedestris voucher UPOL ZL0051 16S ribosomal RNA gene, partial sequence; mitochondrial	409	95.8%	181.0	3.69e-87	82.0%
224	PP474518	Attagenus smirnovi voucher AS18 large subunit ribosomal RNA gene, partial sequence; mitochondrial	401	93.9%	179.0	4.77e-86	82.0%
225	GU356759	Espanoliella jeanneli 16S ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	171.0	1.34e-81	82.0%
226	HG915669	Espanoliella urdialensis mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher IBE	377	88.3%	171.0	1.34e-81	82.0%
227	GU356749	Bathysciola diegoi 16S ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	171.0	1.34e-81	82.0%
228	NC_027176	Luciola substriata mitochondrion, complete genome	377	88.3%	170.0	4.81e-81	82.0%
229	KT303654	Comaserica sp. DA4350 voucher ZFMK:COL:DA4350 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	377	88.3%	169.0	1.73e-80	82.0%
230	AY131448	Cephalodesmius armiger 16S ribosomal RNA gene, partial sequence; mitochondrial	418	97.9%	188.0	4.74e-91	81.9%
231	PQ239537	Chlorophorus annularoides mitochondrion, complete genome	427	100.0%	188.0	4.74e-91	81.9%
232	HG915685	Quaestus amicalis dilatatus mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher IBE	411	96.3%	183.0	2.85e-88	81.9%
233	HE663528	Cantabrogeus sp. 2 IR-2012 mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and nad1 gene (partial), specimen voucher IBE-AC83	411	96.3%	183.0	2.85e-88	81.9%
234	HE663535	Cantabrogeus sp. 1 IR-2012 mitochondrial partial 16S rRNA gene, specimen voucher IBE-RA34	411	96.3%	183.0	2.85e-88	81.9%
235	HE663529	Cantabrogeus sp. 3 IR-2012 mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and nad1 gene (partial), specimen voucher IBE-RA241	411	96.3%	183.0	2.85e-88	81.9%
236	ON386263	Quaestus pseudoccidentalis voucher MNCN-AC64 large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	183.0	2.85e-88	81.9%
237	GU356782	Quaestus jeannei 16S ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	411	96.3%	183.0	2.85e-88	81.9%
238	JN581692	Alevonota rufotestacea voucher ZMUN:10030810 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	408	95.6%	182.0	1.03e-87	81.9%
239	KT696235	Staphylinidae sp. BMNH 1274643 mitochondrion	404	94.6%	179.0	4.77e-86	81.9%
240	MN122896	Anoplotrupes stercorosus mitochondrion	377	88.3%	169.0	1.73e-80	81.9%
241	KT303912	Gynaecoserica aniniensis voucher BMNH:834621 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	377	88.3%	169.0	1.73e-80	81.9%
242	EF487842	Comaserica sp. BMNH 671401 16S large subunit ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	377	88.3%	169.0	1.73e-80	81.9%
243	MT862428	Anoplotrupes stercorosus isolate DM81 mitochondrion	377	88.3%	169.0	1.73e-80	81.9%
244	MT113339	Attagenus augustatus augustatus mitochondrion, complete genome	427	100.0%	190.0	3.66e-92	81.8%
245	MH789722	Anthicidae sp. 1 ACP-2013 mitochondrion	427	100.0%	188.0	4.74e-91	81.8%
246	GQ981045	Philhygra debilis voucher ZMUN:10002608 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	415	97.2%	184.0	7.93e-89	81.8%
247	OR076801	Notriolus sp. 2 MF-2023a voucher H247.3 large subunit ribosomal RNA gene, partial sequence; mitochondrial	414	97.0%	184.0	7.93e-89	81.8%
248	MN473108	Dynamostes audax mitochondrion, partial genome	411	96.3%	181.0	3.69e-87	81.8%
249	MT571474	Troglocharinus jacasi voucher IBE_VR22 large subunit ribosomal RNA (rrnL) and tRNA-Leu (trnL) genes, partial sequence; mitochondrial	411	96.3%	180.0	1.33e-86	81.8%
250	HF912632	Troglocharinus ferreri pallaresi mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher IBE	411	96.3%	180.0	1.33e-86	81.8%
251	HG915637	Anillochlamys moroderi mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher IBE	386	90.4%	172.0	3.72e-82	81.8%
252	KP250244	Anoplotrupes stercorosus voucher SK300 16S ribosomal RNA gene, partial sequence; mitochondrial	386	90.4%	172.0	3.72e-82	81.8%
253	KC992696	Oocyclus pico 16S ribosomal RNA gene, partial sequence	381	89.2%	169.0	1.73e-80	81.8%
254	OW052253	Philonthus cognatus genome assembly, organelle: mitochondrion	415	97.2%	182.0	1.03e-87	81.7%
255	KT303666	Trochalus sp. DA0688 voucher ZFMK:COL:DA0688 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	182.0	1.03e-87	81.7%
256	MH317865	Oocyclus sp. SLE0850 16S ribosomal RNA gene, partial sequence; mitochondrial	415	97.2%	180.0	1.33e-86	81.7%
257	GU356755	Breuilites eloyi 16S ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	411	96.3%	180.0	1.33e-86	81.7%
258	KT304048	Empecamenta sp. 835126 voucher BMNH:835126 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	402	94.1%	178.0	1.72e-85	81.7%
259	KF625954	Lampyridae sp. UPOL RK0378 16S ribosomal RNA gene, partial sequence; mitochondrial	404	94.6%	176.0	2.22e-84	81.7%
260	AB436506	Drilaster axillaris mitochondrial gene for 16S rRNA, partial sequence, isolate: 07706A_a	404	94.6%	176.0	2.22e-84	81.7%
261	KT303997	Empecamenta sp. DA0224 voucher ZFMK:COL:DA0224 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	402	94.1%	176.0	2.22e-84	81.7%
262	KP143636	Afissula sp. KS233 16S ribosomal RNA gene, partial sequence; mitochondrial	386	90.4%	171.0	1.34e-81	81.7%
263	KT445803	Hippodamia variegata voucher Riyadh-2 16S ribosomal RNA gene, partial sequence; mitochondrial	381	89.2%	169.0	1.73e-80	81.7%
264	KT445793	Hippodamia variegata voucher Jeddah-3 16S ribosomal RNA gene, partial sequence; mitochondrial	381	89.2%	169.0	1.73e-80	81.7%
265	KT445791	Hippodamia variegata voucher Jeddah-1 16S ribosomal RNA gene, partial sequence; mitochondrial	381	89.2%	169.0	1.73e-80	81.7%
266	KT303640	Trochalus diversirostris voucher ZFMK:COL:DA1008 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	182.0	1.03e-87	81.6%
267	KY495942	Aenictosymbia cornuta 16S ribosomal RNA gene, partial sequence; mitochondrial	413	96.7%	182.0	1.03e-87	81.6%
268	ON386256	Quaestus acuminatus voucher IBE-AF76 large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	180.0	1.33e-86	81.6%
269	HG915675	Oresigenus jaspei mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher IBE	411	96.3%	180.0	1.33e-86	81.6%
270	HE663531	Cantabrogeus nadali mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and nad1 gene (partial), specimen voucher IBE-AF4	411	96.3%	180.0	1.33e-86	81.6%
271	ON386267	Quaestus spinulosus voucher MNCN-AC66 large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	180.0	1.33e-86	81.6%
272	ON386258	Quaestus jeannei jeannei voucher IBE-AC99 large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	180.0	1.33e-86	81.6%
273	ON386260	Quaestus nuptialis voucher MNCN-AI1079 large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	180.0	1.33e-86	81.6%
274	ON386265	Quaestus sp. 1 AF-2022a voucher IBE-AF77 large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	180.0	1.33e-86	81.6%
275	KP682970	Bruchidius marginalis 16S ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	179.0	4.77e-86	81.6%
276	GQ981030	Atheta contristata voucher ZMUN:10002635 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	402	94.1%	176.0	2.22e-84	81.6%
277	KT780685	Aleocharinae sp. 5 EF-2015 mitochondrion, complete genome	424	99.3%	184.0	7.93e-89	81.5%
278	HQ619674	Colotes punctatus voucher UPOL001171 16S ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	184.0	7.93e-89	81.5%
279	HG915689	Quaestus cisnerosii vasconicus mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher IBE	411	96.3%	180.0	1.33e-86	81.5%
280	KT303815	Trochalus sp. DA1508 voucher ZFMK:COL:DA1508 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	179.0	4.77e-86	81.5%
281	FJ903712	Anthicidae sp. UPOL ZL0063 16S ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	178.0	1.72e-85	81.5%
282	MH317852	Oocyclus itapecori isolate SLE0880 16S ribosomal RNA gene, partial sequence; mitochondrial	414	97.0%	178.0	1.72e-85	81.5%
283	ON386257	Quaestus arcanus voucher IBE-PB32 large subunit ribosomal RNA gene, partial sequence; mitochondrial	409	95.8%	178.0	1.72e-85	81.5%
284	EF570395	Trox arcuatus isolate 69 16S ribosomal RNA gene, partial sequence; mitochondrial	404	94.6%	176.0	2.22e-84	81.5%
285	MH317848	Oocyclus cf. floccus SLE0421 16S ribosomal RNA gene, partial sequence; mitochondrial	405	94.8%	174.0	2.87e-83	81.5%
286	KP682971	Bruchidius pusillus 16S ribosomal RNA gene, partial sequence; mitochondrial	400	93.7%	174.0	2.87e-83	81.5%
287	HQ178231	Bruchidius seminarius voucher BRU-Sc11 16S ribosomal RNA gene, partial sequence; mitochondrial	400	93.7%	173.0	1.03e-82	81.5%
288	AB009928	Drilaster sp. 'Kume-jima Island' mitochondrial DNA for 16S rRNA, partial sequence	404	94.6%	173.0	1.03e-82	81.5%
289	KP682740	Bruchidius incarnatus voucher BRU.I11 16S ribosomal RNA gene, partial sequence; mitochondrial	400	93.7%	172.0	3.72e-82	81.5%
290	KY495944	Anommatoxenus sp. JP-2017 16S ribosomal RNA gene, partial sequence; mitochondrial	389	91.1%	170.0	4.81e-81	81.5%
291	EF487857	Anoplotrupes stercorosus voucher BMNH 703012 16S large subunit ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	386	90.4%	169.0	1.73e-80	81.5%
292	AY131449	Cephalodesmius quadridens 16S ribosomal RNA gene, partial sequence; mitochondrial	416	97.4%	181.0	3.69e-87	81.4%
293	EF487811	Aphodius barbarus 16S ribosomal RNA gene, partial sequence	417	97.7%	180.0	1.33e-86	81.4%
294	EF487829	Aphodius nigrita 16S ribosomal RNA gene, partial sequence	417	97.7%	180.0	1.33e-86	81.4%
295	KT304009	Trochalus sp. DA1656 voucher ZFMK:COL:DA1656 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	179.0	4.77e-86	81.4%
296	KT304075	Trochalus sp. DA1506 voucher ZFMK:COL:DA1506 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	179.0	4.77e-86	81.4%
297	KT303650	Trochalus vagus voucher ZFMK:COL:DA0476 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	179.0	4.77e-86	81.4%
298	ON386261	Quaestus sp. 2 AF-2022a voucher MNCN-AC71 large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	177.0	6.17e-85	81.4%
299	HF912608	Speonomus fagniezi mitochondrial 16S rRNA and tRNA-Leu partial genes, specimen voucher IBE	411	96.3%	177.0	6.17e-85	81.4%
300	AM229401	Speonomus carrerei mitochondrial 16S rRNA gene	411	96.3%	177.0	6.17e-85	81.4%
301	MT571469	Speonomus curvipes curvipes voucher MNCN_HI24 large subunit ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	411	96.3%	177.0	6.17e-85	81.4%
302	ON386266	Quaestus sellai voucher MNCN-AC65 large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	177.0	6.17e-85	81.4%
303	GU356793	Speonomus carrerei 16S ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	411	96.3%	177.0	6.17e-85	81.4%
304	LN849367	Speonomus carrerei genomic DNA containing rrnL-trnL-nad1 region, specimen voucher MNCN-AI527	411	96.3%	177.0	6.17e-85	81.4%
305	JN581725	Geostiba bicarinata voucher ZMUN:10030875 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	406	95.1%	176.0	2.22e-84	81.4%
306	KT303679	Camenta sp. DA3936 voucher ZFMK:COL:DA3936 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	176.0	2.22e-84	81.4%
307	GQ981021	Atheta setigera voucher ZMUN:10002639 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	406	95.1%	175.0	7.99e-84	81.4%
308	MH317864	Oocyclus sp. SLE0852 16S ribosomal RNA gene, partial sequence; mitochondrial	404	94.6%	174.0	2.87e-83	81.4%
309	NC_068852	Stilicoderus aquilinus mitochondrion, complete genome	401	93.9%	173.0	1.03e-82	81.4%
310	AB009927	Drilaster axillaris mitochondrial DNA for 16S rRNA, partial sequence	404	94.6%	173.0	1.03e-82	81.4%
311	GU356760	Espanoliella luquei 16S ribosomal RNA (rrnL) gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	399	93.4%	171.0	1.34e-81	81.4%
312	OQ716344	Heterothops canariensis voucher EH037FAY0255 mitochondrion, partial genome	390	91.3%	170.0	4.81e-81	81.4%
313	KP250268	Phalacrognathus muelleri voucher SK115 16S ribosomal RNA gene, partial sequence; mitochondrial	426	99.8%	184.0	7.93e-89	81.3%
314	KX035165	Hypothenemus sp. BMNH 1039866 mitochondrion, complete genome	426	99.8%	183.0	2.85e-88	81.3%
315	KT303940	Trochalus diversirostris voucher ZFMK:COL:DA0988 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	179.0	4.77e-86	81.3%
316	KP250250	Lamprima adolphinae voucher SK113 16S ribosomal RNA gene, partial sequence; mitochondrial	417	97.7%	178.0	1.72e-85	81.3%
317	ON386262	Quaestus olajensis voucher MNCN-AC63 large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	177.0	6.17e-85	81.3%
318	KT303844	Trochalus sp. DA1519 voucher ZFMK:COL:DA1519 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	176.0	2.22e-84	81.3%
319	KT303767	Trochalus sp. DA1655 voucher ZFMK:COL:DA1655 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	176.0	2.22e-84	81.3%
320	KT303887	Trochalus sp. DA3921 voucher ZFMK:COL:DA3921 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	176.0	2.22e-84	81.3%
321	KT303875	Trochalus sp. DA1499 voucher ZFMK:COL:DA1499 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	176.0	2.22e-84	81.3%
322	DQ198677	Flabellotreta obscuricollis 16S ribosomal RNA gene, partial sequence; mitochondrial	412	96.5%	175.0	7.99e-84	81.3%
323	HQ178230	Bruchidius pygmaeus voucher BRU-Pb11 16S ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	175.0	7.99e-84	81.3%
324	AY847534	Cephalodesmius armiger 16S ribosomal RNA gene, partial sequence; mitochondrial	405	94.8%	174.0	2.87e-83	81.3%
325	KP682975	Bruchus affinis 16S ribosomal RNA gene, partial sequence; mitochondrial	398	93.2%	170.0	4.81e-81	81.3%
326	GQ981042	Lyprocorrhe anceps voucher ZMUN:10002649 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	395	92.5%	170.0	4.81e-81	81.3%
327	AY131520	Macroderes sp. dgi1 16S ribosomal RNA gene, partial sequence; mitochondrial	417	97.7%	180.0	1.33e-86	81.2%
328	AB285542	Silpha tristis mitochondrial gene for 16S ribosomal RNA, partial sequence	419	98.1%	177.0	6.17e-85	81.2%
329	KT304169	Trochalus vagus voucher ZFMK:COL:DA0475 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	176.0	2.22e-84	81.2%
330	KT304068	Trochalus sp. DA3823 voucher ZFMK:COL:DA3823 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	176.0	2.22e-84	81.2%
331	KT304170	Trochalus sp. DA1559 voucher ZFMK:COL:DA1559 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	176.0	2.22e-84	81.2%
332	KT303590	Trochalus vagus voucher ZFMK:COL:DA1289 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	176.0	2.22e-84	81.2%
333	DQ198681	Gorhamia minuta 16S ribosomal RNA gene, partial sequence; mitochondrial	412	96.5%	175.0	7.99e-84	81.2%
334	NC_013554	Adelium sp. NCS-2009 mitochondrion, complete genome	409	95.8%	174.0	2.87e-83	81.2%
335	KP250281	Sinodendron rugosum voucher SK150 16S ribosomal RNA gene, partial sequence; mitochondrial	404	94.6%	172.0	3.72e-82	81.2%
336	NC_047198	Bruchidius uberatus mitochondrion, complete genome	398	93.2%	171.0	1.34e-81	81.2%
337	KP682746	Bruchidius raddianae voucher BRU.R11 16S ribosomal RNA gene, partial sequence; mitochondrial	401	93.9%	171.0	1.34e-81	81.2%
338	MH433166	Ranunculiphilus faeculentus voucher ZFMK:TIS-3472 16S ribosomal RNA gene, partial sequence; mitochondrial	426	99.8%	182.0	1.03e-87	81.1%
339	NC_052914	Spondylis buprestoides mitochondrion, complete genome	427	100.0%	180.0	1.33e-86	81.1%
340	JF796459	Cafius algarum isolate CNUIC-97 16S ribosomal RNA gene, partial sequence; mitochondrial	414	97.0%	176.0	2.22e-84	81.1%
341	MH317870	Oocyclus yubai isolate SLE962 16S ribosomal RNA gene, partial sequence; mitochondrial	415	97.2%	175.0	7.99e-84	81.1%
342	KP682737	Bruchidius guanchorum voucher BRU.GK277 16S ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	175.0	7.99e-84	81.1%
343	ON386264	Quaestus recordationis voucher IBE-AF20 large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	174.0	2.87e-83	81.1%
344	JF796492	Remus corallicola isolate CNUIC-130 16S ribosomal RNA gene, partial sequence; mitochondrial	415	97.2%	174.0	2.87e-83	81.1%
345	ON386268	Quaestus suevensis voucher MNCN-AC67 large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	174.0	2.87e-83	81.1%
346	HG915682	Parapropus sericeus sinuaticollis mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher MNCN:AC52	411	96.3%	174.0	2.87e-83	81.1%
347	LR793121	Ochthebius caudatus mitochondrion genomic DNA containing rrnL region, specimen voucher IBE-AV112	417	97.7%	173.0	1.03e-82	81.1%
348	KP682735	Bruchidius chloroticus voucher BRU.Cc11 16S ribosomal RNA gene, partial sequence; mitochondrial	414	97.0%	173.0	1.03e-82	81.1%
349	AB354089	Epilachna admirabilis mitochondrial gene for 16S ribosomal RNA, partial sequence	402	94.1%	172.0	3.72e-82	81.1%
350	NC_066707	Ocypus graeseri mitochondrion, complete genome	414	97.0%	172.0	3.72e-82	81.1%
351	KP143632	Epilachna admirabilis voucher KS228 16S ribosomal RNA gene, partial sequence; mitochondrial	402	94.1%	172.0	3.72e-82	81.1%
352	AJ841358	Nisotra goudoti mitochondrial partial 16S rRNA gene	404	94.6%	171.0	1.34e-81	81.1%
353	GQ980989	Oxypoda praecox voucher ZMUN:10002637 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	402	94.1%	171.0	1.34e-81	81.1%
354	MH317851	Oocyclus sp. 'imitator' 16S ribosomal RNA gene, partial sequence; mitochondrial	405	94.8%	171.0	1.34e-81	81.1%
355	HQ178236	Bruchidius villosus voucher BRU-V21 16S ribosomal RNA gene, partial sequence; mitochondrial	400	93.7%	169.0	1.73e-80	81.1%
356	HQ619653	Clanoptilus marginellus voucher UPOL001099 16S ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	179.0	4.77e-86	81.0%
357	JF796460	Cafius algophilus isolate CNUIC-98 16S ribosomal RNA gene, partial sequence; mitochondrial	425	99.5%	178.0	1.72e-85	81.0%
358	EF487785	Lichnanthe rathvoni 16S ribosomal RNA gene, partial sequence; mitochondrial	424	99.3%	178.0	1.72e-85	81.0%
359	MH433097	Datonychus maurus voucher ZFMK:TIS-3571 16S ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	178.0	1.72e-85	81.0%
360	KT780629	Atheta gagatina mitochondrion	424	99.3%	177.0	6.17e-85	81.0%
361	MZ571646	Attagenus pellio voucher Leg15 large subunit ribosomal RNA gene, partial sequence; mitochondrial	416	97.4%	176.0	2.22e-84	81.0%
362	MW551203	Coccinella undecimpunctata voucher SJ01-15 large subunit ribosomal RNA gene, partial sequence; mitochondrial	414	97.0%	175.0	7.99e-84	81.0%
363	FM994841	Coccinella undecimpunctata mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher BMNH:673200	414	97.0%	175.0	7.99e-84	81.0%
364	KC992689	Laccobius reflexipenis 16S ribosomal RNA gene, partial sequence	415	97.2%	174.0	2.87e-83	81.0%
365	DQ198678	Flabellotreta sp. UPOL 000M34 16S ribosomal RNA gene, partial sequence; mitochondrial	412	96.5%	174.0	2.87e-83	81.0%
366	AB285549	Oiceoptoma thoracicum mitochondrial gene for 16S ribosomal RNA, partial sequence	414	97.0%	173.0	1.03e-82	81.0%
367	AM287067	Enochrus testaceus mitochondrial partial 16S rRNA gene	412	96.5%	173.0	1.03e-82	81.0%
368	NC_071380	Necrodes nigricornis voucher NFS37 mitochondrion, complete genome	414	97.0%	173.0	1.03e-82	81.0%
369	MH433140	Mogulonoides radula voucher ZFMK:TIS-26016 16S ribosomal RNA gene, partial sequence; mitochondrial	414	97.0%	173.0	1.03e-82	81.0%
370	JX234445	Niphetophora sp. DA-2012 voucher 842732 16S ribosomal RNA gene and tRNA-Leu gene, partial sequence; mitochondrial	411	96.3%	173.0	1.03e-82	81.0%
371	OQ716308	Agathidium sp. voucher SCI2752 mitochondrion, partial genome	411	96.3%	173.0	1.03e-82	81.0%
372	KT780697	Tasgius compressus mitochondrion	414	97.0%	172.0	3.72e-82	81.0%
373	KF625947	Lampyridae sp. UPOL RK0118 16S ribosomal RNA gene, partial sequence; mitochondrial	412	96.5%	172.0	3.72e-82	81.0%
374	KT304139	Pseudotrochalus sp. DA1524 voucher ZFMK:COL:DA1524 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	172.0	3.72e-82	81.0%
375	HG915660	Bathysciotes khevenhuelleri khevenhuelleri mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher IBE	411	96.3%	171.0	1.34e-81	81.0%
376	MH317854	Oocyclus sp. 'latus' 16S ribosomal RNA gene, partial sequence; mitochondrial	405	94.8%	169.0	1.73e-80	81.0%
377	KC524891	Omadius cf. diversipes NLG-2013 voucher 7215 16S ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	180.0	1.33e-86	80.9%
378	NC_085501	Melanotus fiumii mitochondrion, complete genome	427	100.0%	178.0	1.72e-85	80.9%
379	DQ202590	Notoxus monocerus voucher BMNH:679270 16S ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	177.0	6.17e-85	80.9%
380	HQ619660	Cyrtosus semimarginatus voucher UPOL001110 16S ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	176.0	2.22e-84	80.9%
381	JF796493	Remus sericeus isolate CNUIC-131 16S ribosomal RNA gene, partial sequence; mitochondrial	425	99.5%	175.0	7.99e-84	80.9%
382	EF487824	Aphodius nigrivittis 16S ribosomal RNA gene, partial sequence; mitochondrial	417	97.7%	174.0	2.87e-83	80.9%
383	KT303950	Trochalus exasperans voucher ZFMK:COL:DA0790 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	173.0	1.03e-82	80.9%
384	KT303848	Nedymoserica sp. 835140 voucher BMNH:835140 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	173.0	1.03e-82	80.9%
385	OR076762	Hydora annectens voucher H39.1 large subunit ribosomal RNA gene, partial sequence; mitochondrial	414	97.0%	173.0	1.03e-82	80.9%
386	KT303592	Trochalus exasperans voucher ZFMK:COL:DA1282 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	173.0	1.03e-82	80.9%
387	EF487876	Trochalus sp. BMNH 671453 16S large subunit ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	411	96.3%	173.0	1.03e-82	80.9%
388	KT303896	Trochalus sp. DA2928 voucher ZFMK:COL:DA2928 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	173.0	1.03e-82	80.9%
389	LR742657	Oiceoptoma thoracicum mitochondrion genomic DNA containing 16S-tRNA-Leu-NAD6 region, specimen voucher MNCN-AI837	417	97.7%	173.0	1.03e-82	80.9%
390	MH317858	Oocyclus miza isolate SLE0884 16S ribosomal RNA gene, partial sequence; mitochondrial	415	97.2%	172.0	3.72e-82	80.9%
391	KP682755	Kingsolverius malaccanus voucher BRU.Xw11 16S ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	172.0	3.72e-82	80.9%
392	KP682754	Kingsolverius malaccanus voucher BRU.GK150 16S ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	172.0	3.72e-82	80.9%
393	HG915688	Quaestus cantabricus mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher IBE	411	96.3%	171.0	1.34e-81	80.9%
394	KT303571	Gryphonycha puberula voucher ZFMK:COL:DA4365 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	170.0	4.81e-81	80.9%
395	LR793126	Ochthebius sp. IBE-AV149 mitochondrion genomic DNA containing rrnL region, specimen voucher IBE-AV149	417	97.7%	170.0	4.81e-81	80.9%
396	MN885946	Astathes sp. IBE JGZ-3734 voucher IBE-JGZ-3734 large subunit ribosomal RNA gene, partial sequence; mitochondrial	409	95.8%	169.0	1.73e-80	80.9%
397	JN581759	Tetradonia sp. 1 HE-2012 voucher ZMUN:10029163 16S ribosomal RNA gene, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and NADH dehydrogenase subunit 1 (NADH1) gene, partial cds; mitochondrial	405	94.8%	169.0	1.73e-80	80.9%
398	ON797419	Riolus subviolaceus isolate Rsubv2 large subunit ribosomal RNA gene, partial sequence; mitochondrial	404	94.6%	169.0	1.73e-80	80.9%
399	OM868038	Morimospasma tuberculatum mitochondrion, complete genome	399	93.4%	169.0	1.73e-80	80.9%
400	NC_067970	Sclerotia fui mitochondrion, complete genome	424	99.3%	175.0	7.99e-84	80.8%
401	HQ178227	Bruchidius murinus voucher BRU-Ma11 16S ribosomal RNA gene, partial sequence; mitochondrial	425	99.5%	175.0	7.99e-84	80.8%
402	FM994760	Coccinella undecimpunctata mitochondrial 16S rRNA gene (partial), tRNA-Leu gene and NAD1 gene (partial), specimen voucher BMNH:668336	414	97.0%	173.0	1.03e-82	80.8%
403	DQ202544	Attagenus pellio voucher BMNH:679216 16S ribosomal RNA gene, partial sequence; mitochondrial	416	97.4%	173.0	1.03e-82	80.8%
404	AJ862736	Limnius perrisi partial mitochondrial 16S rRNA gene	417	97.7%	173.0	1.03e-82	80.8%
405	KY495941	Aenictophila sp. JP-2017 16S ribosomal RNA gene, partial sequence; mitochondrial	415	97.2%	171.0	1.34e-81	80.8%
406	EF532581	Plateumaris metallica isolate N1469 16S ribosomal RNA gene, partial sequence; mitochondrial	409	95.8%	170.0	4.81e-81	80.8%
407	AB285537	Oiceoptoma subrufum mitochondrial gene for 16S ribosomal RNA, partial sequence	414	97.0%	170.0	4.81e-81	80.8%
408	KT303825	Trochalus sp. DA3049 voucher ZFMK:COL:DA3049 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	170.0	4.81e-81	80.8%
409	OR901692	Mogulonoides radula isolate Mradu_Z28490 mitochondrion, complete genome	411	96.3%	170.0	4.81e-81	80.8%
410	KT303684	Camenta sp. 835173 voucher BMNH:835173 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	170.0	4.81e-81	80.8%
411	JX234523	Heterorrhina cf. sexmaculata DA-2012 voucher 842849 16S ribosomal RNA gene and tRNA-Leu gene, partial sequence; mitochondrial	411	96.3%	170.0	4.81e-81	80.8%
412	KC992668	Enochrus fimbriatus 16S ribosomal RNA gene, partial sequence	412	96.5%	170.0	4.81e-81	80.8%
413	AB285545	Silpha imitator mitochondrial gene for 16S ribosomal RNA, partial sequence	414	97.0%	170.0	4.81e-81	80.8%
414	OY720324	Philonthus spinipes genome assembly, organelle: mitochondrion	407	95.3%	169.0	1.73e-80	80.8%
415	MH317853	Oocyclus itapecum isolate SLE0874 16S ribosomal RNA gene, partial sequence; mitochondrial	414	97.0%	169.0	1.73e-80	80.8%
416	MN885932	Colobothea leucophaea voucher IBE-JGZ-3702 large subunit ribosomal RNA gene, partial sequence; mitochondrial	424	99.3%	175.0	7.99e-84	80.7%
417	AJ781540	Promecosoma viride partial 16S rRNA gene	424	99.3%	175.0	7.99e-84	80.7%
418	MH433047	Ceutorhynchus pyrrhorhynchus canariensis voucher ZFMK:TIS-23883 16S ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	175.0	7.99e-84	80.7%
419	AY131539	Eurysternus plebejus 16S ribosomal RNA gene, partial sequence; mitochondrial	417	97.7%	174.0	2.87e-83	80.7%
420	KT780627	Atheta sp. 1 EF-2015 mitochondrion, partial genome	424	99.3%	174.0	2.87e-83	80.7%
421	MF286007	Agrilus nicolanus voucher UPOL EJ0295 16S ribosomal RNA gene, partial sequence; tRNA-Leu gene, complete sequence; and NADH dehydrogenase subunit 1 (ND1) gene, partial cds; mitochondrial	427	100.0%	172.0	3.72e-82	80.7%
422	MF521888	Batocera lineolata voucher KM20150516 mitochondrion, complete genome	411	96.3%	171.0	1.34e-81	80.7%
423	NC_022671	Batocera lineolata mitochondrion, complete genome	411	96.3%	171.0	1.34e-81	80.7%
424	KP133973	Silvanus muticus 16S ribosomal RNA gene, partial sequence; mitochondrial	414	97.0%	170.0	4.81e-81	80.7%
425	AM287071	Cercyon ustulatus mitochondrial partial 16S rRNA gene	412	96.5%	170.0	4.81e-81	80.7%
426	KT304000	Trochalus sp. DA3889 voucher ZFMK:COL:DA3889 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	170.0	4.81e-81	80.7%
427	KF737740	Rhaphuma elongata voucher HNAU.PP 024 16S ribosomal RNA gene, partial sequence; mitochondrial	408	95.6%	169.0	1.73e-80	80.7%
428	LR793119	Ochthebius sp. IBE-AV163 mitochondrion genomic DNA containing rrnL-trnL-NAD1 region, specimen voucher IBE-AV163	414	97.0%	169.0	1.73e-80	80.7%
429	KT303892	Trochalus sp. DA0991 voucher ZFMK:COL:DA0991 16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	411	96.3%	169.0	1.73e-80	80.7%
430	GQ302155	Sphaerosoma sp. 1 16S ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	175.0	7.99e-84	80.6%
431	MW365444	Cordylepherus sp. YY-2021 mitochondrion, complete genome	427	100.0%	174.0	2.87e-83	80.6%
432	AJ495493	Metacinops rhinomacer partial mitochondrial 16S rRNA gene	425	99.5%	173.0	1.03e-82	80.6%
433	NC_084303	Sclerotia aquatilis voucher KU-2013-35-02 mitochondrion, complete genome	424	99.3%	172.0	3.72e-82	80.6%
434	MT012203	Cafius maritimus isolate Jimmy Armers beach, KA, New Zealand large subunit ribosomal RNA gene, partial sequence; mitochondrial	425	99.5%	172.0	3.72e-82	80.6%
435	OM688363	Spondylis buprestoides voucher KOU-SSSB382 large subunit ribosomal RNA gene, partial sequence; mitochondrial	410	96.0%	171.0	1.34e-81	80.6%
436	JF796487	Philonthus nudus isolate CNUIC-125 16S ribosomal RNA gene, partial sequence; mitochondrial	425	99.5%	171.0	1.34e-81	80.6%
437	OM688362	Spondylis buprestoides voucher KOU-SSSB379 large subunit ribosomal RNA gene, partial sequence; mitochondrial	410	96.0%	171.0	1.34e-81	80.6%
438	OM688366	Spondylis buprestoides voucher KOU-SSSB21 large subunit ribosomal RNA gene, partial sequence; mitochondrial	410	96.0%	171.0	1.34e-81	80.6%
439	KF737757	Batocera horsfieldi voucher HNAU.PP 041 16S ribosomal RNA gene, partial sequence; mitochondrial	414	97.0%	171.0	1.34e-81	80.6%
440	OM688364	Spondylis buprestoides voucher KOU-SSSB23 large subunit ribosomal RNA gene, partial sequence; mitochondrial	410	96.0%	171.0	1.34e-81	80.6%
441	OM688361	Spondylis buprestoides voucher KOU-SSSB24 large subunit ribosomal RNA gene, partial sequence; mitochondrial	410	96.0%	171.0	1.34e-81	80.6%
442	OM688367	Spondylis buprestoides voucher KOU-SSSB22 large subunit ribosomal RNA gene, partial sequence; mitochondrial	410	96.0%	171.0	1.34e-81	80.6%
443	OR076766	Hydora sp. 7 MF-2023a voucher H75.2 large subunit ribosomal RNA gene, partial sequence; mitochondrial	414	97.0%	170.0	4.81e-81	80.6%
444	DQ266475	Limnius intermedius 16S ribosomal RNA gene, partial sequence; mitochondrial	417	97.7%	170.0	4.81e-81	80.6%
445	ON210290	Morimospasma sp. mitochondrion, complete genome	416	97.4%	170.0	4.81e-81	80.6%
446	OR076759	Hydora sp. 8 MF-2023a voucher H9.3 large subunit ribosomal RNA gene, partial sequence; mitochondrial	414	97.0%	170.0	4.81e-81	80.6%
447	NC_067895	Morimospasma tuberculatum mitochondrion, complete genome	416	97.4%	170.0	4.81e-81	80.6%
448	OR076769	Hydora sp. 5 MF-2023a voucher H88.1 large subunit ribosomal RNA gene, partial sequence; mitochondrial	414	97.0%	169.0	1.73e-80	80.6%
449	KF625964	Lampyridae sp. UPOL RK0388 16S ribosomal RNA gene, partial sequence; mitochondrial	412	96.5%	169.0	1.73e-80	80.6%
450	AJ231143	Timarcha granadensis mitochondrial 16S rRNA gene, partial	427	100.0%	174.0	2.87e-83	80.5%
451	PP209352	Wagnerinus harmandi isolate Wharm_J1 mitochondrion, complete genome	426	99.8%	172.0	3.72e-82	80.5%
452	MN420476	Spondylis buprestoides mitochondrion, complete genome	425	99.5%	172.0	3.72e-82	80.5%
453	OQ716371	Othius sp. voucher LG380EHA0803 mitochondrion, partial genome	424	99.3%	172.0	3.72e-82	80.5%
454	JX982941	Pelidnota frommeri 16S ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	172.0	3.72e-82	80.5%
455	MH433078	Ceutorhynchus pyrrhorhynchus voucher ZFMK:TIS-3195 16S ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	172.0	3.72e-82	80.5%
456	DQ198633	Paralichas pectinatus 16S ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	171.0	1.34e-81	80.5%
457	KP829024	Cycloxenus sp. CO924 16S ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	171.0	1.34e-81	80.5%
458	AJ231139	Timarcha cyanescens mitochondrial 16S rRNA gene, partial	414	97.0%	169.0	1.73e-80	80.5%
459	AJ781539	Percolaspis pulchella partial 16S rRNA gene	424	99.3%	172.0	3.72e-82	80.4%
460	MT701170	Bothrometopus huntleyi isolate Bhu_MA02 16S ribosomal RNA gene, partial sequence; mitochondrial	426	99.8%	172.0	3.72e-82	80.4%
461	OZ177486	Anthocomus fasciatus genome assembly, organelle: mitochondrion	427	100.0%	171.0	1.34e-81	80.4%
462	PP203206	Prisistus suturalba isolate Psutu_Z69589 mitochondrion, complete genome	426	99.8%	171.0	1.34e-81	80.4%
463	MH433136	Microplontus millefolii voucher ZFMK:TIS-3711 16S ribosomal RNA gene, partial sequence; mitochondrial	426	99.8%	171.0	1.34e-81	80.4%
464	MZ488576	Pieleus irregularis mitochondrion, complete genome	427	100.0%	171.0	1.34e-81	80.4%
465	AJ231160	Timarcha sp. DEH-1995 partial mitochondrial 16S rRNA gene, strain Leon	427	100.0%	171.0	1.34e-81	80.4%
466	PV026027	Batocera calanus mitochondrion, complete genome	425	99.5%	171.0	1.34e-81	80.4%
467	AJ622034	Timarcha rugulosa lomnickii mitochondrial partial 16S rRNA gene, country Poland:Kazimier Dolny	427	100.0%	171.0	1.34e-81	80.4%
468	OP747313	Curtos sp. voucher KU-2016-05-51 mitochondrion, complete genome	424	99.3%	169.0	1.73e-80	80.4%
469	MW858150	Aulaconotus atronotatus mitochondrion, partial genome	426	99.8%	172.0	3.72e-82	80.3%
470	MN885986	Nyctimenius ochraceovittatus voucher IBE-JGZ-3783 large subunit ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	172.0	3.72e-82	80.3%
471	MF975129	Timarcha balearica voucher TimBal16S large subunit ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	171.0	1.34e-81	80.3%
472	AJ231136	Timarcha aurichalcea mitochondrial 16S rRNA gene, partial	427	100.0%	171.0	1.34e-81	80.3%
473	AJ622029	Timarcha erosa vermiculata mitochondrial partial 16S rRNA gene, country Portugal:Porto Alto, Santarem	427	100.0%	171.0	1.34e-81	80.3%
474	AJ236387	Timarcha balearica mitochondrial 16S rRNA gene (partial), isolate Puig de Randa, Mallorca, Spain	427	100.0%	171.0	1.34e-81	80.3%
475	MF975018	Oomela distincta voucher JAJ87 large subunit ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	171.0	1.34e-81	80.3%
476	KC524898	Omadius mediofaciatus voucher 8106 16S ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	171.0	1.34e-81	80.3%
477	MF974937	Calomela maculicollis voucher JAJ10 large subunit ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	171.0	1.34e-81	80.3%
478	AJ236383	Timarcha hispanica mitochondrial 16S rRNA gene (partial), isolate Puerto de Caracuel, Ciudad Real, Spain	427	100.0%	171.0	1.34e-81	80.3%
479	AJ236376	Timarcha perezi mitochondrial 16S rRNA gene (partial), isolate Puerto de Linares, Teruel, Spain	427	100.0%	171.0	1.34e-81	80.3%
480	MZ490582	Clerus dealbatus mitochondrion, complete genome	426	99.8%	170.0	4.81e-81	80.3%
481	AJ236382	Timarcha hispanica mitochondrial 16S rRNA gene (partial), isolate Sao Torpes beach, Alentejo, Portugal	427	100.0%	170.0	4.81e-81	80.3%
482	MF975043	Peltoschema festiva voucher JAJ45 large subunit ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	170.0	4.81e-81	80.3%
483	OY757319	Stenurella melanura genome assembly, organelle: mitochondrion	425	99.5%	170.0	4.81e-81	80.3%
484	MZ475053	Clerus klapperichi mitochondrion, partial genome	426	99.8%	170.0	4.81e-81	80.3%
485	MZ464017	Callimerus inbasalis mitochondrion, partial genome	426	99.8%	170.0	4.81e-81	80.3%
486	MG988489	Cymatodera mexicana 16S ribosomal RNA gene, partial sequence; mitochondrial	426	99.8%	170.0	4.81e-81	80.3%
487	KC524907	Natalis sp. 1 NLG-2013 voucher 8208 16S ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	169.0	1.73e-80	80.3%
488	AJ622030	Timarcha maritima mitochondrial partial 16S rRNA gene, country France:Le Canon, Aquitaine	427	100.0%	171.0	1.34e-81	80.2%
489	AY131625	Sisyphus faciculatus 16S ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	170.0	4.81e-81	80.2%
490	AJ236370	Timarcha interstitialis mitochondrial 16S rRNA gene (partial), isolate Caramany, France	427	100.0%	170.0	4.81e-81	80.2%
491	KU739490	Euoniticellus intermedius mitochondrion	424	99.3%	169.0	1.73e-80	80.2%
492	MF974978	Dicranosterna immaculata voucher 196156 large subunit ribosomal RNA gene, partial sequence; mitochondrial	426	99.8%	169.0	1.73e-80	80.2%
493	MF974979	Dicranosterna immaculata voucher 196157 large subunit ribosomal RNA gene, partial sequence; mitochondrial	426	99.8%	169.0	1.73e-80	80.2%
494	NC_053714	Aromia bungii mitochondrion, complete genome	427	100.0%	170.0	4.81e-81	80.1%
495	HQ832601	Aromia bungii voucher CNE1031 16S ribosomal RNA gene, partial sequence; mitochondrial	427	100.0%	170.0	4.81e-81	80.1%
496	MF975081	Paropsides sp. 4 JAJR-2017 voucher JAJ212 large subunit ribosomal RNA gene, partial sequence; mitochondrial	426	99.8%	170.0	4.81e-81	80.1%
497	AJ236369	Timarcha interstitialis mitochondrial 16S rRNA gene (partial), isolate Garraf, Barcelona, Spain	427	100.0%	169.0	1.73e-80	80.1%
498	MT701172	Bothrometopus huntleyi isolate Bhu_PE01 16S ribosomal RNA gene, partial sequence; mitochondrial	426	99.8%	169.0	1.73e-80	80.1%
499	MT701174	Bothrometopus huntleyi isolate Bhu_PE03 16S ribosomal RNA gene, partial sequence; mitochondrial	426	99.8%	169.0	1.73e-80	80.1%
500	NC_080229	Batocera horsfieldi isolate tissue mitochondrion, complete genome	426	99.8%	169.0	1.73e-80	80.0%

Selected alignment

Selected taxonomy

Kingdom
Phylum
Class
Order
Family
Genus
Species

Analyst evaluation

Taxa of interest

This sections shows the taxa of interest specified by the submitter. Each of these taxa has been cross-referenced against the candidate species to determine if they might match the taxonomic identity of the sample. A blank row indicates a TOI that did not match any candidate species, meaning that it is unlikely that the sample matches that TOI.

Taxon of interest	Match rank	Match taxon	Match species	Match accession	Match identity
Trogoderma granarium	species	Trogoderma granarium	Trogoderma granarium	MZ571637	1.0

See the Database coverage section to see database coverage for taxa of interest.

Reference sequence source diversity

This analysis evaluates how many independent sources have contributed to reference sequences for each candidate species. This provides a measure of confidence in the taxonomic annotation of references sequences. A sequence that has been annotated by multiple independent sources is more likely to have a correct taxonomic annotation.

Analyst evaluation

Candidates

Independent sources

Flag 4A: Reference sequences are from diverse sources and therefore likely to be reliable
Reasoning: Matching sequence records for this species are from >5 independent sources (found 6 sources)

6 Independent Sources

The matching reference sequences for this species have been annotated by 6 independent source(s). A source is considered independent if the author list or publication title is distinct.

Source 1

Hit accession	Automated	Authors	Title	Journal
MZ571637	False	Rako,L. Agarwal,A. Semeraro,L. Broadley,A. Rodoni,B.C. Blacket,M.J.	A LAMP (loop-mediated isothermal amplification) test for rapid identification of Khapra beetle (Trogoderma granarium)	Pest Manag Sci (2021) In press
MZ571637	False	Blacket,M. Rako,L. Agarwal,A.	Direct Submission	Submitted (13-JUL-2021) AVR, Agriculture Victoria, AgriBio, Bundoora, Victoria 3083, Australia
MZ571636	False	Rako,L. Agarwal,A. Semeraro,L. Broadley,A. Rodoni,B.C. Blacket,M.J.	A LAMP (loop-mediated isothermal amplification) test for rapid identification of Khapra beetle (Trogoderma granarium)	Pest Manag Sci (2021) In press
MZ571636	False	Blacket,M. Rako,L. Agarwal,A.	Direct Submission	Submitted (13-JUL-2021) AVR, Agriculture Victoria, AgriBio, Bundoora, Victoria 3083, Australia

Source 2

Hit accession	Automated	Authors	Title	Journal
KJ930431	False	Olson,R.L.O. Farris,R.E. Barr,N.B. Cognato,A.I.	Molecular identification of Trogoderma granarium (Coleoptera: Dermestidae) using the 16S gene	Unpublished
KJ930431	False	Olson,R.L.O. Farris,R.E. Barr,N.B. Cognato,A.I.	Direct Submission	Submitted (02-JUN-2014) Center for Learning Innovation, University of Minnesota, Rochester, 111 South Broadway Suite 300, Rochester, MN 55904, USA
KJ930432	False	Olson,R.L.O. Farris,R.E. Barr,N.B. Cognato,A.I.	Molecular identification of Trogoderma granarium (Coleoptera: Dermestidae) using the 16S gene	Unpublished
KJ930432	False	Olson,R.L.O. Farris,R.E. Barr,N.B. Cognato,A.I.	Direct Submission	Submitted (02-JUN-2014) Center for Learning Innovation, University of Minnesota, Rochester, 111 South Broadway Suite 300, Rochester, MN 55904, USA
KJ930452	False	Olson,R.L.O. Farris,R.E. Barr,N.B. Cognato,A.I.	Molecular identification of Trogoderma granarium (Coleoptera: Dermestidae) using the 16S gene	Unpublished
KJ930452	False	Olson,R.L.O. Farris,R.E. Barr,N.B. Cognato,A.I.	Direct Submission	Submitted (02-JUN-2014) Center for Learning Innovation, University of Minnesota, Rochester, 111 South Broadway Suite 300, Rochester, MN 55904, USA
KJ930433	False	Olson,R.L.O. Farris,R.E. Barr,N.B. Cognato,A.I.	Molecular identification of Trogoderma granarium (Coleoptera: Dermestidae) using the 16S gene	Unpublished
KJ930433	False	Olson,R.L.O. Farris,R.E. Barr,N.B. Cognato,A.I.	Direct Submission	Submitted (02-JUN-2014) Center for Learning Innovation, University of Minnesota, Rochester, 111 South Broadway Suite 300, Rochester, MN 55904, USA

Source 3

Hit accession	Automated	Authors	Title	Journal
OM388538	False	mzhr,N.N. Jawad,M.M. Sabr,A.J.	Direct Submission	Submitted (25-JAN-2022) biology, Baghdad university, palestine street, baghdad, baghgdad 10046, Iraq
OM389182	False	mzhr,N.N. Jawad,M.M. Sabr,A.J.	Direct Submission	Submitted (25-JAN-2022) biology, Baghdad university, palestine street, baghdad, baghgdad 10046, Iraq
OM388512	False	Mzhr,N.N. Jawad,M.M. Sabr,A.J.	Direct Submission	Submitted (25-JAN-2022) biology, Baghdad university, palestine street, baghdad, baghgdad 10046, Iraq
OM388567	False	Mzhr,N.N. Jawad,M.M. Sabr,A.J.	Direct Submission	Submitted (25-JAN-2022) biology, Baghdad university, palestine street, baghdad, baghgdad 10046, Iraq
OM389131	False	Mzhr,N.N. Jawad,M.M. Sabr,A.J.	Direct Submission	Submitted (25-JAN-2022) biology, Baghdad university, palestine street, baghdad, baghgdad 10046, Iraq

Source 4

Hit accession	Automated	Authors	Title	Journal
LC386208	False	Furui,S. Miyanoshita,A. Imamura,T. Minegishi,Y. Kokutani,R.	Qualitative real-time PCR identification of the khapra beetle, Trogoderma granarium (Coleoptera: Dermestidae)	Appl. Entomol. Zool. (Jpn.) 54, 101-107 (2019)
LC386208	False	Furui,S. Minegishi,Y. Kokutani,R.	Direct Submission	Submitted (28-MAY-2018) Contact:Satoshi Furui Food Research Institute, NARO, Food Entomology Laboratory; 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan URL :http://www.naro.affrc.go.jp/english/index.html

Source 5

Hit accession	Automated	Authors	Title	Journal
ON725093	False	Kavallieratos,N.G. Boukouvala,M.C. Lorentha Gidari,D. Di Giuseppe,G. Canale,A. Benelli,G.	Does cross-mating affect behavioral asymmetries and mating success of Trogoderma granarium strains?	Unpublished
ON725093	False	Di Giuseppe,G.	Direct Submission	Submitted (10-JUN-2022) Department of Biology, University of Pisa, Italy, Via A. Volta, 4, Pisa, PI I-56126, Italy

Source 6

Hit accession	Automated	Authors	Title	Journal
NC_053875	False	Zeng,L. Pang,Y. Feng,S. Wang,Y. Stejskal,V. Aulicky,R. Zhang,S. Li,Z.	Comparative mitochondrial genomics of five Dermestid beetles (Coleoptera: Dermestidae) and its implications for phylogeny	Genomics (2020) In press
NC_053875	False		Direct Submission	Submitted (25-MAR-2021) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
NC_053875	False	Zeng,L. Pang,Y. Li,Z.	Direct Submission	Submitted (25-FEB-2020) Department of Entomology, College of Plant Protection, China Agricultural University, Yuanmingyuan West Road 2#, Haidian District, Beijing 100193, China

Database coverage of target taxa

The target taxa include candidate species, the preliminary morphology ID, and any taxa of interest provided by the submitter. Each of these taxa are independently evaluated against the reference database to determine whether sufficient reference data exists to support identification of the target taxon. Insufficient coverage of a taxon can result in that taxon not be correctly identified as the taxonomic identity of the sample. For example, if the sample is Homo sapiens, but Homo sapiens sequences are not included in the reference database, the analysis will be unable to identity Homo sapiens as the correct taxonomic identity, and will most likely assign the closest relative with reference data as the taxonomic identity.

Analyst evaluation

Candidates

Preliminary ID

Taxa of interest

Database coverage

Flag 5.1C: The reference database is likely to be unreliable for this species
Reasoning: The given locus for this taxon is not present in reference database (0 entries)

Database coverage of Trogoderma granarium

Flag 5.1C: The reference database is likely to be unreliable for this species
Reasoning: The given locus for this taxon is not present in reference database (0 entries)

0 records

There are 0 sequences in the reference database for Trogoderma granarium at the given locus 16S mitochondrial rRNA gene.

Database coverage of species in genus Trogoderma

Flag 5.2C: The database has poor support for species in this genus
Reasoning: ≤10% of taxon have reference sequence(s) at the given locus

/ (%) sequence records were found in the reference database for:

Species in the genus Trogoderma
At the target locus 16S mitochondrial rRNA gene

Number of GenBank records at locus 16S mitochondrial rRNA gene

Database coverage of species in genus Trogoderma that occur in country of origin

Flag 5.3C: Probability that a different related species from the country of origin is the true taxonomic identity: LOW
Reasoning: No species in genus have been observed in the country of origin

/ (%) sequence records were found in the reference database for:

Species in the genus Trogoderma
At the target locus 16S mitochondrial rRNA gene
In the sample country of origin Pakistan

Number of GenBank records at locus 16S mitochondrial rRNA gene

Database coverage

Flag 5.1C: The reference database is likely to be unreliable for this species
Reasoning: The given locus for this taxon is not present in reference database (0 entries)

Database coverage of Trogoderma

Flag 5.1C: The reference database is likely to be unreliable for this species
Reasoning: The given locus for this taxon is not present in reference database (0 entries)

0 records

There are 0 sequences in the reference database for Trogoderma at the given locus 16S mitochondrial rRNA gene.

Database coverage of species in genus Trogoderma

Flag 5.2C: The database has poor support for species in this genus
Reasoning: ≤10% of taxon have reference sequence(s) at the given locus

/ (%) sequence records were found in the reference database for:

Species in the genus Trogoderma
At the target locus 16S mitochondrial rRNA gene

Number of GenBank records at locus 16S mitochondrial rRNA gene

Database coverage of species in genus Trogoderma that occur in country of origin

Flag 5.3A: Probability that a different related species from the country of origin is the true taxonomic identity: LOW
Reasoning: All species in genus from country of origin have reference sequence(s) for this locus

/ (%) sequence records were found in the reference database for:

Species in the genus Trogoderma
At the target locus 16S mitochondrial rRNA gene
In the sample country of origin Pakistan

Number of GenBank records at locus 16S mitochondrial rRNA gene

Database coverage

Flag 5.1C: The reference database is likely to be unreliable for this species
Reasoning: The given locus for this taxon is not present in reference database (0 entries)

Database coverage of Trogoderma granarium

Flag 5.1C: The reference database is likely to be unreliable for this species
Reasoning: The given locus for this taxon is not present in reference database (0 entries)

0 records

There are 0 sequences in the reference database for Trogoderma granarium at the given locus 16S mitochondrial rRNA gene.

Database coverage of species in genus Trogoderma

Flag 5.2C: The database has poor support for species in this genus
Reasoning: ≤10% of taxon have reference sequence(s) at the given locus

/ (%) sequence records were found in the reference database for:

Species in the genus Trogoderma
At the target locus 16S mitochondrial rRNA gene

Number of GenBank records at locus 16S mitochondrial rRNA gene

Database coverage of species in genus Trogoderma that occur in country of origin

/ (%) sequence records were found in the reference database for:

Species in the genus Trogoderma
At the target locus 16S mitochondrial rRNA gene
In the sample country of origin Pakistan

Number of GenBank records at locus 16S mitochondrial rRNA gene

Intraspecies diversity

This section provides a phylogeny of the candidate reference sequences. The analyst can use this to make a subjective observation on how well the reference sequences are able to distinguish between species. If the phylogeny shows distinct clades for each species, we can be confident that the molecular data are capable of distinguishing between those species. However, if the phylogeny shows overlap between species, this reduces the capacity of the molecular data to confidently distinguish between those species. In some cases, we may see the query sequence falling outside of the adjacent species' clades, which indicates that our query species is not represented in the reference database, which could indicate a rare or novel species.

Analyst evaluation

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The phylogetic tree was constructed with FastME using the Neighbor-Joining method. Multiple-sequence alignment of the candidate reference sequences was performed using MAFFT. The visualization is rendered with TidyTree.

Taxonomy Check

The following resources can be used to ensure that the given taxonomy is legitimate and current.

Taxa	Database
General	GBIF
General	ITIS
Mealybugs & scale	ScaleNet database
Thrips	Thripswiki
Spider Mites	Spider Mites Database
Psocodea (Barklice, Booklice, and Parasitic Lice)	Psocodea Species File Online
Orthoptera	Orthoptera Species File Online
Drosophilidae	TaxoDros
Diptera	Catalog of the Diptera of the Australasian and Oceanian Regions Systema Dipterorum
Aphids	Aphid Species File
Ants	AntWeb AntCat
Lepidoptera (butterflies and moths)	The Global Lepidoptera Names Index
Gracillariidae (primitive moths)	Global Taxonomic Database of Gracillariidae
Pyralidae (pyralid moths)	Global Information System on Pyraloidea
Tortricidae (tortrix moths)	Tortricidae Resources on the Net

Taxonomic assignment report

Sample details

Input DNA sequence

Result overview

Analyst evaluation

Identification of candidate species

BLAST search results

Analyst evaluation

Taxa of interest

Reference sequence source diversity

Analyst evaluation

Database coverage of target taxa

Analyst evaluation

Database coverage of Trogoderma granarium

Database coverage of species in genus Trogoderma

Per-species reference sequence counts

Database coverage of species in genus Trogoderma that occur in country of origin

Per-species reference sequence counts

Database coverage of Trogoderma

Database coverage of species in genus Trogoderma

Per-species reference sequence counts

Database coverage of species in genus Trogoderma that occur in country of origin

Per-species reference sequence counts

Database coverage of Trogoderma granarium

Database coverage of species in genus Trogoderma

Per-species reference sequence counts

Database coverage of species in genus Trogoderma that occur in country of origin

Per-species reference sequence counts

Intraspecies diversity

Analyst evaluation

Taxonomy Check